Columbus Crew secured a Wild Card berth in the Eastern Conference with a 2-0 victory over FC Dallas. Midfielder Santiago Rodríguez contributed a goal and assist, while goalkeeper Patrick Schulte recorded seven saves. Charlotte FC clinched their third consecutive playoff spot under Dean Smith, with Liel Abada scoring a hat trick off the bench in a 4-0 demolition of CF Montréal.

New England Revolution strengthened their hold on the East's second seed with a 3-2 win over Red Bull New York. Carles Gil became the eighth MLS player to reach 100 regular-season assists and set up striker Dor Turgeman for two goals. Vancouver Whitecaps moved closer to the West's top seed after defeating San Diego FC 4-1, with Ryan Gauld registering one goal and one assist.

Antoine Griezmann extended his streak to 12 consecutive matches with a goal contribution in Orlando City's 2-2 draw at Chicago Fire FC. Kristoffer Velde scored twice to help Portland Timbers defeat St. Louis CITY SC 3-2. Houston Dynamo's Lucas Halter scored his first MLS goal in a 1-0 win at Seattle Sounders FC, while Minnesota United's Anthony Markanich netted in a 3-1 comeback victory over Sporting Kansas City.